Universal Mycoplasma Detection Kit from American Type Culture Collection (ATCC)

Universal Mycoplasma Detection Kit from American Type Culture Collection (ATCC)

Universal Mycoplasma Detection Kit from American Type Culture Collection (ATCC)

Description

The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or E.coli, is not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.

The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma, including the top eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.

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  • Detailed Specifications
  • ItemUniversal Mycoplasma Detection Kit
  • CompanyAmerican Type Culture Collection (ATCC)
  • PriceGet Quote
  • Catalog Number30-1012K
  • Regulatory ComplianceMeets European Pharmacopeia 2.6.7 guidelines
  • Pharmaceutical ApplicationRoutine testing of cell cultures
  • MethodPCR
  • SensitivityInquire
  • Analysis TimeLess than 3 hours
American Type Culture Collection (ATCC)
American Type Culture Collection (ATCC)
P.O. Box 1549
Manassas, VA 20108
United States
Phone: (703) 365-2700
Website: www.atcc.org